PCR from dried sequencing gel?

Michael Cooley szcooley at chip.ucdavis.edu
Thu Nov 17 02:35:19 EST 1994


schupp at nauvax.ucc.nau.edu wrote:
: Hello,
:         Has anyone tried to perform PCR on bands excised from dried
: Sequencing gels?

: Jim Schupp

Yes, but only if it was not fixed. We just boiled the band in TE for 15 
minutes and precipitated using linear polyacrylamide as carrier. Dissolve 
the pellet in 10 ul TE and use 3 ul in the PCR reaction.


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