Using Glycogen to prec DNA???

[Michael Cooley]

Seanna Annis (sannis at uoguelph.ca) wrote:
: I am trying to purify a PCR fragments, around 600 bp from gel.  I can get 
: a decent amount out of the gel, but I lose it during the precipitation 
: step.  I want to use this product for direct cycle sequencing, so yeast 
: tRNA as an aid to precipitating the DNA was suggested to me to be a bad idea.
: I have heard of glycogen used to prec oligonucleotides.  Does anyone know 
: of the right concentration of glycogen to use.  And does it interfer with 
: sequencing afterwards?  Or does anyone know of another method to get a 
: better yield.  
: Thanks.  Seanna    (sannis at uoguelph.ca)

I have used linear acrylamide and it works fine and doesn't interfer with 
PCR and it's cheap. The suggested method is to use 10 - 20 ug of linear 
acrylamide in the precipitation. See NAR 18:378 for the method on how to 
make it. 


#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%
%                                                        #
#   Dr. Michael Cooley         Practice Random Kindness  %
%   mbcooley at ucdavis.edu    and Senseless Acts of Beauty #
#                                                        %
%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#