unable to ppt. protein using ammonium sulphate

Tim Buss tbuss at fred.fhcrc.org
Thu Nov 17 20:46:01 EST 1994


The ability to precipitate protein with ammonium sulphate depends alot on
its (the protein's) concentration. If it is quite dilute it may never
precipitate out, no matter how much ammonium sulphate you add. Also, the
percent saturation of ammonium sulphate needed for a particular protein
to precipitate will vary depending on its concentration.

Tim.



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