Platinum/Biotin complex

Hugues Roest Crollius hrc at gea.lif.icnet.uk
Thu Nov 17 07:20:14 EST 1994


Hello all,


I have recently heard of a new system to label DNA and RNA with biotin,
 which involves a platinum/biotin compound. In a 30 min incubation at 80 degrees C,
 this complex binds to guanin groups, with no need for enzymes etc...

Has anybody used this system to label DNA in applications such as DNA hybridisation
on nylon membranes, and could provide me with some hints on the subject
(i.e. incorporation, stability, background on filter, etc ..) ?

Many thanks in advance for any help.


Hugues Roest Crollius
Genome Analysis Lab
ICRF, London
hrc at gea.lif.icnet.uk



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