Nylon membrane blues

Sasha Kraev bckraev at aeolus.ethz.ch
Thu Nov 17 14:38:05 EST 1994


Hi, Julie 
First, yes, blotting from 1.4% gel is somewhat inefficient. However,
you did not made yourself clear enough with respect to blotting details.
1. How long are the fragments you want to see?
2. What is the trasnfer setup?
3. Do you run a copy reconstruction control for actual sensitivity?
I can recommend UV crosslinking, since under optimal conditions it substantia-
lly increases sensitivity. Hope this helps.



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