FLAGged Protein Purification
Seth M Fisher
Seth_M_Fisher%notes at sb.com
Thu Nov 17 14:25:52 EST 1994
>Is the chaperonin binding directly to your M2 gel or interacting with
>your recombinant protein?
Based on gel filtration under 'native' conditions chaperonin was not associated
with the protein I was attempting to purify. I called the manafacturer about
cross-reactivity of the M2 and they said,' Oh, was it chaperonin?'. So, I think
this problem is known to them.
> Ihave not used FLAG in bacteria, but have used it effectively in both
>mammalian cells and insect cells.
I am about to purify a FLAGged protein from yeast and I will let you know how
it went. I may have to try mammalian or insect cells.
More information about the Methods