Nylon membrane blues
jwilber at pop.nd.edu
Thu Nov 17 11:59:49 EST 1994
I am having problems getting good sgnals on DNAse I hypersensitive assays.
I suspect that it may be blotting. I alkaline blot onto Nylon membrane
from biorad (zeta probe). Is there anything about the gel (1.4%), or DNA
sample that might be causing this. Even my parent fragment is coming up
light. I load 20 ug of DNA per lane. After blotting I neutralize for 10
minutes in 50mM Tris pH 7.5 and then bake for about 45 minutes. I AM
Can anyone offer any clues or advice? Thanks!
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