Sequencing Gel Fixation

Anthony Tomlinson tomlinson at pplros.demon.co.uk
Thu Nov 17 11:18:18 EST 1994


In article <tsute.267.000B56F6 at microbio.umass.edu> Tsute Chen,
tsute at microbio.umass.edu writes:
>I've heard that the fixation step (with 10% acetic acid & 10% methanol)
can be 
>skipped without much difference in the result during the normal Sanger's
DNA 
>sequencing procedures. Does anyone have any experience or opinion about
this?

I remember that some people said they had problems in
humid environments with unfixed gels, but most seemed
to feel that fixing sould be omitted if you're in a hurry.
 I've not had the nerve yet!



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