Sequencing Gel Fixation

Anthony Tomlinson tomlinson at
Thu Nov 17 11:18:18 EST 1994

In article <tsute.267.000B56F6 at> Tsute Chen,
tsute at writes:
>I've heard that the fixation step (with 10% acetic acid & 10% methanol)
can be 
>skipped without much difference in the result during the normal Sanger's
>sequencing procedures. Does anyone have any experience or opinion about

I remember that some people said they had problems in
humid environments with unfixed gels, but most seemed
to feel that fixing sould be omitted if you're in a hurry.
 I've not had the nerve yet!

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