Sequencing Gel Fixation

[Anthony Tomlinson]

In article <tsute.267.000B56F6 at microbio.umass.edu> Tsute Chen,
tsute at microbio.umass.edu writes:
>I've heard that the fixation step (with 10% acetic acid & 10% methanol)
can be 
>skipped without much difference in the result during the normal Sanger's
DNA 
>sequencing procedures. Does anyone have any experience or opinion about
this?

I remember that some people said they had problems in
humid environments with unfixed gels, but most seemed
to feel that fixing sould be omitted if you're in a hurry.
 I've not had the nerve yet!