pH management

[Robert Preston]

In article <9411181327.aa24926 at cbdcom.apgea.army.mil>, 
rxyoung at CBDA9.APGEA.ARMY.MIL (Ronald Young) wrote:

> There is a large drop in pH in the enzyme reaction I am studying that 
> cannot be controlled by the buffer in use because of the large release 
> in H+....I am sure others have faced this problem and I would appreciate any 
> suggestions.

Hmmm.  Even 50 mM buffer at it's pK should sop up 10 mM H+ with no drastic
pH change.
You must need to make molar amounts of product for some reason???  OK,
throw in some 
limestone and put it under partial vacuum to suck off CO2.  Course, you'd
have to tolerate 
Ca2+ in amounts half as much as the protons you generate, but I guess .1
Molar Ca2+ 
isn't as nasty as .2M H+, as a rule.

-- 
Robert Preston                                    rapr at med.pitt.edu
U. Pittsburgh Pathology Dept.                   Pittsburgh PA 15261
vox 412-648-9573                                   fax 412-648-1916
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