dchar at cns.neusc.bcm.tmc.edu
Tue Nov 15 10:12:17 EST 1994
Any body has experience of inducing "primer dimerization"? . I am using PCR to dectect my
insert in CHO cell. The cell also has the endogenous gene. Since there are introns, the length of
endogenous gene as I predicted is above 1 kb. while the length of the 2 exons without intron is
less than 300 base. I need to show that this 300 base band is not "primer dimerizatin artifact".
Somebody told me to add 200ug BSA will help. Thanx in advance.
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