PCR

David Char dchar at cns.neusc.bcm.tmc.edu
Tue Nov 15 10:12:17 EST 1994


Any body has experience of inducing "primer dimerization"? .  I am using PCR to dectect my 
insert in CHO cell.  The cell also has the endogenous gene.  Since there are introns, the length of 
endogenous gene as I predicted is above 1 kb.  while the length of the 2 exons without intron is 
less than 300 base.  I need to show that this 300 base band is not "primer dimerizatin artifact".  
Somebody told me to add 200ug BSA will help.   Thanx in advance. 



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