RNA-Isolation from plant seeds using CTAB

J. Lang btb501 at btrtx1.uni-bayreuth.de
Fri Nov 18 12:44:20 EST 1994


Hello evrywhere,
Has anybody some experience using CTAB buffers for isolation of RNAs from plant seeds
or any other tissues.
My problem is, that I only get genomic DNA and appearently tRNAs but no rRNAs or
mRNAs (which I need most) using a buffer with CTAB (3%), 1.4 M NaCl, EDTA 20 mM,
beta- Mercaptoethanol (2%), ProteinaseK, Tris 100mM. Does anybody know whether it 
depends on the salt concentration if the mRNAs, rRNAs and tRNAs precipitate or not.
Thanx a lot
please write to jens.lang at btr0x1.hrz.uni-bayreuth.de



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