plasmid prep with G+ bacteria
kannan at kuhub.cc.ukans.edu
kannan at kuhub.cc.ukans.edu
Fri Nov 18 18:17:42 EST 1994
Does anyone out there know how to purify plasmids from Gram + bacteria--
i want purify a plasmid from drug resistant Staph. sp. to see if the plasmid is
responsible for imparting resistance. The Promega miniprep kit doesn't work.
i also tried to treat the cells with Lysostaphin for 15 min. and then did a
wizard miniprep. it didn't work either. i always see a smear on the gel.
anyinput on this sue would be of great help.
Thanks.
Kannan Thiru, University of Kansas.
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