plasmid prep with G+ bacteria

kannan at kannan at
Fri Nov 18 18:17:42 EST 1994

Does anyone out there know how to purify plasmids from Gram + bacteria--
i want purify a plasmid from drug resistant Staph. sp. to see if the plasmid is 
responsible for imparting resistance.  The Promega miniprep kit doesn't work.  
i also tried to treat the cells with Lysostaphin for 15 min. and then did a 
wizard miniprep.  it didn't work either.  i always see a smear on the gel.
anyinput on this sue would be of great help.
Kannan Thiru, University of Kansas.

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