Nightmare with GST fusion protein

[T. S. Pillay]

Subject: Nightmare with GST fusion protein
From: Jean-Yves Masson, massonjy at rsvs.ulaval.ca
Date: 18 Nov 1994 19:45:39 -0800
In article <Pine.3.89.9411182228.A2259-0100000 at eukaryota> Jean-Yves
Masson, massonjy at rsvs.ulaval.ca writes:
>
>Dear netters,
>
>        Since two months, no matter what I do, my GST fusion protein is 
>degraded in E. coli. I tried the following , etc.


-Is AD202  a protease deficient strain?  If not, have you tried such a
beast.
- How about growing at room temp.?
- 3rdly, ( I'm sure you have checked this by sequencing) but could this
have a premature stop codon.