?best purification of 186bp DNA fragment from agaraose

Robert C. Colgrove robin at alumni.caltech.edu
Mon Nov 21 13:13:41 EST 1994


I am cloning a small (186bp) PCR product. Usually I clone larger fragments
and purify the gel fragments with "geneclean." Their package insert
suggests not using it for less than 200bp. In the old days, I used all
the standard tricks: Low melt agarose, electroelution, mashing up the 
gel slice, etc. I was just wondering what people tihink is the best way
to go about it these days. Thanks.
					robin colgrove




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