Lac Operon Mutants

Ann Kleinschmidt akleinsc at bio7.alleg.edu
Mon Nov 21 12:30:58 EST 1994


Howdy,

In our introductory biology laboratory we do an experiment in which  
we analyze the effect of glucose and lactose on b-galactosidase  
levels.  This then leads into a discussion about the lac operon and  
how these sugars have an effect at the level of transcription.   
Following the initial experiment we have the students design and  
carry out an independent project to further characterize the  
functioning of the lac operon.  For their independent projects, we  
would like to have them look at various lac operon mutants and we  
currently have on hand a strain that has a represser protein that is  
unable to bind to the operator.  We would like to get a wider array  
of mutants, but we don't know the strain names and likely sources for  
these mutants.  Any assistance on this would be appreciated.

Also, does anyone have a protocol that would allow students to  
mutagenize bacteria and select for those that contain lac operon  
mutations?

Thanks for your help.

Responses can be sent directly to:

Ann Kleinschmidt
akleinsc at alleg.edu

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