RNA storage in guanidinium isothiocyanate

Tracy Aquilla aquilla at salus.med.uvm.edu
Mon Nov 21 10:55:55 EST 1994


In Article <Pine.SGI.3.91.941119193133.5646A-100000 at helix.nih.gov>,
rhewitt at HELIX.NIH.GOV (Robert Hewitt) wrote:
>Dear Internetter,
>
>Can anyone tell me whether RNA is stable at -70 degrees C in 
>guanidinium isothiocyanate solution?  
>
>I have been using the Stratagene micro RNA preparation kit for preparation
>of total RNA.  This includes a denaturing solution containing guanidinium,
>which is applied to the cells in the first step of the protocol.  For
>convenience, it would be helpful if the cell lysate + guanidinium could be
>stored at -70 after the first step. Any advice would be much appreciated.
>
>Robert Hewitt
>
>

I have stored cell lysates in GTC solutions at -80C for over a year with no
RNA degradation or other problems. I just take them out of the freezer and
purify the RNA as I need it.
    Tracy



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