best way to remove PCR primers

Jim Owens jow at helix.nih.gov
Mon Nov 21 14:23:52 EST 1994


In article <3ai6ih$phk at lyra.csx.cam.ac.uk> Charlie Wright,
cw117 at mole.bio.cam.ac.uk writes:
>Any other ideas (EtOH precipitation?)

It is claimed that primers do not precipitate well when ethanol
precipitated from 2.5M ammonium acetate.  I use this method routinely
with satisfactory, but not perfect results:

Add 1/3 volume of 10M ammonium acetate, mix, then precipitate with 1.6
volumes of ethanol.  I found this in a Biotechniques article four or five
years ago.

Good luck,

Jim Owens



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