In vitro translation kit?
BARRY J MARGULIES
bjmarg at welchlink.welch.jhu.edu
Tue Nov 22 18:43:23 EST 1994
In article <Stephen_Lasky-2111940922330001 at poncho-slip3.cis.brown.edu>,
Stephen R. Lasky, Ph.D. <Stephen_Lasky at brown.edu> wrote:
>In article <1994Nov18.104548.8772 at mbcf>, neale at mbcf.stjude.org wrote:
>> Can anyone recommend a reliable kit for in vitro transcription/translation?
>> We have our clone under control of T7 RNA promoter.
>> Thanks in advance,
>> Geoff Neale
>The Promega TnT kit works quite nicely although I believe it is more
>efficient from an SP6 promoter than from a T7 promoter.
Hmmm... I would be careful when saying that. With my particular clones,
T7 works a lot better than SP6. The bottom line is that T7 v. SP6 is
entirely empirical, and which one works better depends on the particular
transcript you are trying to make.
-Barry (bjmarg at welchlink.welch.jhu.edu)
GEORGIA CONFEDERATE SOLDIERS from a monument at Antietam
National Battlefield, just south
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