best way to remove PCR primers

Paul N Hengen pnh at
Tue Nov 22 16:18:43 EST 1994

 In article <paquetty.785535111 at alize.ERE.UMontreal.CA>
 paquetty at ERE.UMontreal.CA (Paquette Yves) writes:

> If your PCR fragment is longer than 200 bp, you can also use Geneclean.
> Anything below that size does not bind to the glass particles;
.                          ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> you can wash the glass several times to get rid of all the remaining
> primers (I have tested it with 32P labelled primers) and elute
> your clean PCR fragment.

I don't think this is right. Primers and short ds fragments bind to the glass
particles, but are not eluted very efficiently. If you've done this with
32-P labelled primers, where did the radioactive label end up in the end?

* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
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