best way to remove PCR primers

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Tue Nov 22 16:18:43 EST 1994


 In article <paquetty.785535111 at alize.ERE.UMontreal.CA>
 paquetty at ERE.UMontreal.CA (Paquette Yves) writes:

> If your PCR fragment is longer than 200 bp, you can also use Geneclean.
> Anything below that size does not bind to the glass particles;
.                          ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> you can wash the glass several times to get rid of all the remaining
> primers (I have tested it with 32P labelled primers) and elute
> your clean PCR fragment.

I don't think this is right. Primers and short ds fragments bind to the glass
particles, but are not eluted very efficiently. If you've done this with
32-P labelled primers, where did the radioactive label end up in the end?

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