best way to remove PCR primers

Paquette Yves paquetty at ERE.UMontreal.CA
Tue Nov 22 15:11:51 EST 1994


Jim Owens <jow at helix.nih.gov> writes:

>In article <3ai6ih$phk at lyra.csx.cam.ac.uk> Charlie Wright,
>cw117 at mole.bio.cam.ac.uk writes:
>>Any other ideas (EtOH precipitation?)

If your PCR fragment is longer than 200 bp, you can also use Geneclean.
Anything below that size does not bind to the glass particles;
you can wash the glass several times to get rid of all the remaining
primers (I have tested it with 32P labelled primers) and elute
your clean PCR fragment.

Good luck !

Yves Paquette



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