HELP! Lane background in Northern's

Gil kgilbert at cmp.hmc.psu.edu
Tue Nov 22 15:17:06 EST 1994


Here are some more specifics  which may help someone with my background
problem:

I run a 1.2% agarose/0.4M formaldehyde gel; downward capillary transfer
in 10X SSPE overnight; membrane is washed in 5X SSPE @ 60 degrees for 5
min, then baked @80 degrees for 1 hr, and UV crosslinked in a
Stratalinker (200 ujX100).  

After O/N hybridization, washes are as follows:
1. Quick rinse in 2X SSPE
2. 2 x 15 min washes in 2XSSPE/0.5%SDS at hybridization temperature

KA Gilbert, Ph.D.
Dept. Cell and Mol Physiol
Penn State Univ, Col of Med
Hershey, PA 17033
Voice: 717-531-8997
FAX:   717-531-7667
e-mail: kgilbert at cmp.hmc.psu.edu



More information about the Methods mailing list