Altered Sites II Mutagenesis

Michael J. McLeish Michael_M at
Wed Nov 23 01:22:13 EST 1994

We intend to carry out a series of mutations using this kit, which has come 
with good recommendations from the net.  It seems the protocol calls for 
competent cells prepared using rubidium chloride.  Does anyone know why 
rubidium chloride is necessary?  Is there some advantage over calcium 
chloride alone?

Thanks in advance.


Michael J. McLeish Ph.D.                 :  Michael_m at
Department of Medicinal Chemistry
Victorian College of Pharmacy 
Monash University
Parkville VIC 3052

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