RNA extraction and homogenization

Fredrik Kamme fkhero at biogwn.wblab.lu.se
Wed Nov 23 12:32:10 EST 1994


Hi,
I've used Chomczynski's method to extract RNA from brain successfully,
but, when I try combining it with Qiagen's RNeasy spin columns, the
filter clogs. Can I use an ultrasonic homogenizer instead of a piston to
improve homogeniztion, or will I shear the RNA?
Thanx for any suggestions, Freddy



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