Circular DNA and Stables

[David J. Austin, Ph.D.]

I would like to ask a question for which I am having a hard time finding 
an answer to.  I was told that it was not possible to generate stable 
mammalian cell lines with circular plasmid DNA, that it should be linearized 
first.  Is there a reason for this?

                        -Dave

David J. Austin, Ph.D.      
Department of Chemistry     austin at slsiris.harvard.edu
Harvard University
Cambridge, MA 02138
(617)495-8393
(617)495-0751