Circular DNA and Stables

David J. Austin, Ph.D. austin at SLSIRIS.HARVARD.EDU
Wed Nov 23 13:06:48 EST 1994

I would like to ask a question for which I am having a hard time finding 
an answer to.  I was told that it was not possible to generate stable 
mammalian cell lines with circular plasmid DNA, that it should be linearized 
first.  Is there a reason for this?


David J. Austin, Ph.D.      
Department of Chemistry     austin at
Harvard University
Cambridge, MA 02138

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