[Q] cycle sequencing kits?

Bob Belas belas at mbimail.umd.edu
Thu Nov 24 09:14:35 EST 1994


   I'm in need of advice regarding kits for cycle sequencing using 35S and 
plasmids with large (14 kb) inserts.  It seems to me that this much DNA is 
pushing the envelope of PCR to the limits.  Does anyone have any experience 
with sequencing inserts of such large size?  Although I routinely read 
b.m.m-r, please (if possible) reply directly to me email address.  Thank you 
in advance.

               Bob


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~ "Now imagine a Moebius vortex inside a spherical constant, and you've ~~
~~               got my cosmology."  Frank Zappa, 1940-1993              ~~
~~        Bob Belas, Asst. 



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