[Q] cycle sequencing kits?
belas at mbimail.umd.edu
Thu Nov 24 09:16:34 EST 1994
In article <belas.386.00093E81 at mbimail.umd.edu> belas at mbimail.umd.edu (Bob Belas) writes:
>From: belas at mbimail.umd.edu (Bob Belas)
>Subject: [Q] cycle sequencing kits?
>Date: Thu, 24 Nov 1994 09:14:35
>Organization: Center of Marine Biotechnology, U of MD Bio. Inst.
>Message-ID: <belas.386.00093E81 at mbimail.umd.edu>
>X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]
> I'm in need of advice regarding kits for cycle sequencing using 35S and
>plasmids with large (14 kb) inserts. It seems to me that this much DNA is
>pushing the envelope of PCR to the limits. Does anyone have any experience
>with sequencing inserts of such large size? Although I routinely read
>b.m.m-r, please (if possible) reply directly to me email address. Thank you
>~~ "Now imagine a Moebius vortex inside a spherical constant, and you've ~~
>~~ got my cosmology." Frank Zappa, 1940-1993 ~~
>~~ Bob Belas, Asst.
P.S. I don't know why, but my sig died, along with my email address,
which is BELAS at MBIMAIL.UMD.EDU. Sorry bout that.
~~ "Now imagine a Moebius vortex inside a spherical constant, and you've ~~
~~ got my cosmology." Frank Zappa, 1940-1993 ~~
~~ Bob Belas, Asst.
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