[Q] cycle sequencing kits?

Bob Belas belas at mbimail.umd.edu
Thu Nov 24 09:16:34 EST 1994


In article <belas.386.00093E81 at mbimail.umd.edu> belas at mbimail.umd.edu (Bob Belas) writes:
>Path: umd5.umd.edu!comb15.UMD.EDU!belas
>From: belas at mbimail.umd.edu (Bob Belas)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: [Q] cycle sequencing kits?
>Date: Thu, 24 Nov 1994 09:14:35
>Organization: Center of Marine Biotechnology, U of MD Bio. Inst.
>Lines: 14
>Message-ID: <belas.386.00093E81 at mbimail.umd.edu>
>NNTP-Posting-Host: comb15.umd.edu
>X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]


>   I'm in need of advice regarding kits for cycle sequencing using 35S and 
>plasmids with large (14 kb) inserts.  It seems to me that this much DNA is 
>pushing the envelope of PCR to the limits.  Does anyone have any experience 
>with sequencing inserts of such large size?  Although I routinely read 
>b.m.m-r, please (if possible) reply directly to me email address.  Thank you 
>in advance.

>               Bob


>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>~~ "Now imagine a Moebius vortex inside a spherical constant, and you've ~~
>~~               got my cosmology."  Frank Zappa, 1940-1993              ~~
>~~        Bob Belas, Asst. 


     P.S.   I don't know why, but my sig died, along with my email address, 
which is BELAS at MBIMAIL.UMD.EDU.  Sorry bout that.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~ "Now imagine a Moebius vortex inside a spherical constant, and you've ~~
~~               got my cosmology."  Frank Zappa, 1940-1993              ~~
~~        Bob Belas, Asst. 



More information about the Methods mailing list