how to make sigle-stranded DNA of pET

Yee-Yung Charng szcharng at peseta.ucdavis.edu
Wed Nov 23 14:47:32 EST 1994


Hi, I wonder if anybody can give me a clue to make ssDNA of pET-based
plasmid with good yield.  We have tried Novablue and XL1-blue as host 
cells and USC-M13 as a helper phage without success. I was told to use 
Promega's R408 helper phage instead.  Any input ?  Thanks in advance.

Yee-yung Charng
yccharng at ucdavis.edu  



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