Hi, I wonder if anybody can give me a clue to make ssDNA of pET-based
plasmid with good yield. We have tried Novablue and XL1-blue as host
cells and USC-M13 as a helper phage without success. I was told to use
Promega's R408 helper phage instead. Any input ? Thanks in advance.
Yee-yung Charng
yccharng at ucdavis.edu
