Luciferase detection

Enrico Tongiorgi enrico at genes.icgeb.trieste.it
Thu Nov 24 06:20:21 EST 1994


Hallo everybody,

I transfected HeLa cells with a construct having the Luciferase gene as
a reporter gene. I would like to detect the positive clones directly in
the culture dish by adding the substrata (D-Luciferin etc..).
Has anyone done it ?
Does this procedure kill the cells ?
Can you detect the positive cells in situ ?

Thanks a lot for the help.
Enrico (Trieste. Italy)
e-mail adress: TONGI at SISSA.IT



More information about the Methods mailing list