Direct cloning of a bacterial gene

Michael Cooley szcooley at chip.ucdavis.edu
Tue Nov 22 13:06:08 EST 1994


Shula Zhang (shzhang at uoguelph.ca) wrote:
: Hi, netters:
: Would anyone there be willing to share your ideas and/or experience on 
: how to directly clone a bacterial gene from its genome, whose sequence 
: information is available only in few other related species. Any comments 
: are appreciated.

I cloned directly into Agrobacterium by making a plasmid library and 
electroporating it in. It was easy, but the efficiency of electroporation 
in Agrobacterium is high.


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