Cleaning Electroporation Cuvettes

[Michael Cooley]

chouy at TCPLINK.NREL.GOV wrote:
:      I used to rinse the cuvette with sterile ddH2O three times followed by 
:      filling in with ethanol for until next use.  Right before I use the 
:      cuvette, I then rinse with sterile ddH2O for a couple of times.  It 
:      worked just fine for Lactobacillus electroporation.
:      
:      
:      YC


: ______________________________ Reply Separator _________________________________
: Subject: Cleaning Electroporation Cuvettes
: Author:  ianjf at unixg.ubc.ca (Ian James Forsythe) at smtp
: Date:    94/11/18 15:09


: Can anyone tell me a good way of cleaning electroporation cuvettes for 
: re-use?  Must get rid of all salt and also any residual DNA.
:      
: --
: Ian J. Forsythe, Agriculture & Agrifood Canada
: 6660 NW Marine Drive, Vancouver, BC V6T 1X2  Canada (Campus Zone 4) 
: Telephone:  (604) 224-4355 local 229   Fax:  (604) 666-4994
: e-mail:  ianjf at unixg.ubc.ca
:      

Just rinsing with water is insufficient provided you are using 
electroporation to clone. Residual DNA will appear in subsequent 
electroporations. Treat the cuvettes with 30% HNO3 for 1 hr after rinsing 
with water. Then rinse with water again and fill with 95% EtOH. I just 
let it dry down, but have also dried under vacuum after 24 hrs.


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