discrepancies between cDNA and protein sequences

[Chris Upton]

In Article <3atqps$dfc at jhunix1.hcf.jhu.edu>, ntheo at welchlink.welch.jhu.edu
(NICHOLAS THEODORAKIS ) wrote:
>In article <3aqa0d$ad2 at news.univ-rennes1.fr>,
>Le Pennec Jean-Paul <Lepennec at univ-rennes1.fr> wrote:
>>I have analysed a cDNA sequence of a protein already sequenced by 
>>chemical-physical analysis and I get differences of the amino acids 
>>composition. I'd like  to know wether i can trust 100 % the protein 
>>analysis or go back to my sequence.What do you think? I am looking 
>>forward hearing from you !
>

You don't mention exactly where the differences are......

But, what about splice variants? Perhaps your particular clone is not the
sole product of mRNA splicing.

Cheers,
  Chris  Upton



 Chris Upton  
Biochemistry & Microbiology
University of Victoria
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