discrepancies between cDNA and protein sequences
cupton at sol.uvic.ca
Thu Nov 24 13:40:04 EST 1994
In Article <3atqps$dfc at jhunix1.hcf.jhu.edu>, ntheo at welchlink.welch.jhu.edu
(NICHOLAS THEODORAKIS ) wrote:
>In article <3aqa0d$ad2 at news.univ-rennes1.fr>,
>Le Pennec Jean-Paul <Lepennec at univ-rennes1.fr> wrote:
>>I have analysed a cDNA sequence of a protein already sequenced by
>>chemical-physical analysis and I get differences of the amino acids
>>composition. I'd like to know wether i can trust 100 % the protein
>>analysis or go back to my sequence.What do you think? I am looking
>>forward hearing from you !
You don't mention exactly where the differences are......
But, what about splice variants? Perhaps your particular clone is not the
sole product of mRNA splicing.
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