Mouse chromosomal location blots--NEEDED
takeshtk at is.nyu.edu
Thu Nov 24 23:24:11 EST 1994
In article <3aviu3$5qg at lyra.csx.cam.ac.uk> (bionet.molbio.methds-reagnts),
wmr at mole.bio.cam.ac.uk (William Milford Rideout III (Wellcome-CRC)) wrote:
> I have recently cloned a small PCR product and am interested in quickly
> determining its chromosomal location in the mouse genome.
> I'm looking for a company that sells southern blots of somatic cell
> hybrid-lines which contain single mouse chromosomes in a hamster background.
> This would allow determination of chromosomal location with a single
> hybridization--very quick! Please e-mail addresses or phone numbers of
> any companies which sell such things.
> William Rideout
We have had good luck with mapping using hybrid panels from BIOS company,
New Have, Connecticut, USA. They sell hybrid panels suitable for mapping
using PCR and by the standard southern blot method.
K. Takeshita, NYU Medical Center
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