PCR trouble: inverted repeat template

NAMRATA SRIVASTAVA 5gf4srivasta at vms.csd.mu.edu
Thu Nov 24 11:38:11 EST 1994


Hi netters!
	I've been trying to PCR a 4 Kb construct which has a 1.6 Kb inverted 
repeat in it. I never get the expected product instead I get a smaller product I think the inverted repeat forms a palindrome on itself and the Taq jumps over the palindrome to form the first few products which then get amplified.

	Is there any way to reduce secondary structure in the template? I have
tried a hot start and adding gene 32 protein. Does not seem to help much.
Any suggestions?
		
			Thanking you in advance.

				-Namrata.



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