UV-light for DNA linking and destruction
M O L B I O L
molbiol at cl.nioo.nl
Thu Nov 24 10:37:14 EST 1994
Can anyone give me some explanation as to the proper wavelength for the
1) DNA cross-linking to nylon-membranes. There seem to be at least two
wavelengths in use for commercial cross-linkers 254 and 312 nm. The goal
naturally is to achieve the utmost efficiency in binding and high specificity
in subsequent hybridization.
2) Destruction of DNA. At several places I want to clean the working hood or
labtable, and instruments like pipettes from DNA which would otherwise
contaminate my PCR. However, I can imagine that the shorter wavelengths
deteriorate all sorts of plastics and paint most. Furthermore, I like to now
if perspex is totally effective in protecting me and my fellow-workers from
UV of the proposed wavelengths.
More information about the Methods