mRNA capping kits

[Otto Froehlich]

Andreas Buhr (Buhr at pki.unibe.ch) wrote:

(some stuff deleted)

: What was your yield of RNA? With the CAP kit of Stratagene usualy I get
: 20-30 ug RNA using 2 ug plasmid in 50 yl. This equals the amount of NTP's,
: I think. 
: A friend in Seattle told me  Ambion has problems with its kit at this
: moment. 
: please drop me a line and forward interesting responses to your question.

: Andreas

: buhr at pki.unibe.ch

Dear Andreas,
could you be more specific about the problems with Ambion's kit?
I just spent a few hundred dollars on it and would like to know what I 
need to watch out for. 
Thanks, Otto

--
Otto Froehlich                      tel. (404) 727-7417
Dept. Physiology                    FAX  (404) 727-2648
Emory University                    froehlich at physio.emory.edu
Atlanta, GA 30322                   ofroehl at emoryu1.cc.emory.edu