wizard dna clean up

[Michael Cooley]

PBS::NELSONJ (@PBS.DFO.CA) wrote:
: Hi,
: 	I've recently switched to using the wizard dna clean up system
: for purifying fragments from lmp agarose gels.  Typically I Etoh precip.
: the dna to concentrate it after eluting from the column.  I have been
: having problems getting the dna into solution after this precipitation.
: Has anyone else had this problem or noticed any particularities after
: purifying dna from lmp agarose using the wizard system?
: John Nelson
: nelsonj at pbs.dfo.ca


Are you sure its the DNA that wouldn't go into solution? Promega has been 
going through periods with these columns. Some of them leak the resin. I 
suggest you ignor the pellet and check the super on a gel.


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