cDNA libraries primed with specific probes

J. David Spafford jspaffor at
Sun Nov 27 15:59:10 EST 1994

I am trying to create a specific library (really a specific word) using
positive DNA probes to prime the reverse transcription of mRNA into cDNA. 
How large should the oligonucleotide probes be?  Can the primers be
degenerate?  Is it necessary to spike the library with oligodT or random
primers?  How much polyA+ RNA is it necessary to start with?  

If there are reference, I would appreciate them.

Thanks in advance,

J. David Spafford
Department of Biological Sciences, University of Alberta
jspaffor at

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