32P into Supercoiled -No way ???

Paul Kitts 71774.1102 at CompuServe.COM
Mon Nov 28 23:47:50 EST 1994


I don't know why you need supercoiled plasmid but I was in a lab 
where the postdoc, Evelyn Richet, developed an indirect labelling 
method to do footprinting on supercoiled DNA. The footprinting 
reaction was done on cold sc plasmid, then the DNA was linearized 
and run on a sequencing gel. The DNA was transferred to a filter & 
probed with a labelled oligo that annnealed to one end of the DNA. 
She got nice footprints.
I can dig up the references if you are interested.

-= Paul =- 
[These are my opinions, and not necessarily CLONTECH's]

Paul Kitts                   Email: paulki at clontech.com
CLONTECH laboratories



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