DNase FOOTPRINTING Off a Gel-Retarded Complex

Thiruvamoor P. Ramkumar ramu at artsci.wustl.edu
Mon Nov 28 15:23:58 EST 1994

	i'm looking for help on doing DNase Footprinting from a 
DNA-Protein complex isolated from a gel-retarded complex. Primarily, my 
concern is to elute the DNA-Protein complex out of the gel without 
disrupting the complex. Possible buffer compositions, elution conditions, 
quantitation parameters etc. will all help. 


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