DNA from paraffin-tissue?
bghallor at unmc.edu
Mon Nov 28 14:04:02 EST 1994
Can anyone tell me if DNA can be extracted from paraffin-embedded
tissue? (and no Jurassic Park jokes, please). I have multiple
samples of tissue that were formalin-fixed and embedded in
paraffin for histology.
My questions are: 1) can DNA be extraced 2) is this going to
give me sufficient quantity of DNA (don't need much since it's
for simple gel electrophoresis) 3) is the DNA of decent quality.
If you have a protocol, I would appreciate getting some help.
Maniatis/Current Protocols had nothin'.
Please e-mail responses to
bghallor at unmcvm.unmc.edu
Thanks in advance.
University of Nebraska Medical Center
P.S. Go 'Huskers!
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