Tue Nov 29 12:39:33 EST 1994
In article <1994Nov22.121739.1633014380 at msmail.mpi.com>, Patrick_Kleyn at MSMAIL.MPI.COM says:
>I'm interested in comparing various methods of mutation detection (SSCP,
>DGGE, chemical cleavage, enzymatic). I would like to use some well-defined
>controls: I would like to generate sets of four PCR fragments of identical
>length and sequence except for one particular nucleotide position at which
>each fragment would have one of the four possible bases. Ideally I would
>like to study the effect of local sequence surrounding the mutated base and
>so I'm looking for several such sets with different sequence. Does anyone
>know of or has anyone generated any such constructs as part of a
>mutagenesis experiment ?
>We are trying to produce the same thing, i.e. a Mutant PCR fragment with
altered restriction sites. We have previously generated such a molecule
of 120 bp by using an ABI synthesizer, but we were unsuccessful with 180
bp and above. This appears to be due to the limits of the synthesis.
Any suggestions anybody?
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