Cleaning Electroporation Cuvettes

Eric Kofoid kofoid at biology.utah.edu
Tue Nov 29 11:59:52 EST 1994


In article <3atboq$pfq at mark.ucdavis.edu>, szcooley at chip.ucdavis.edu
(Michael Cooley) wrote:

-chouy at TCPLINK.NREL.GOV wrote:
-:      I used to rinse the cuvette with sterile ddH2O three times followed by 
-:      filling in with ethanol for until next use.  Right before I use the 
-:      cuvette, I then rinse with sterile ddH2O for a couple of times.  It 
-:      worked just fine for Lactobacillus electroporation.
-:      
-:      
-:      YC
-
-
-: ______________________________ Reply Separator
_________________________________
-: Subject: Cleaning Electroporation Cuvettes
-: Author:  ianjf at unixg.ubc.ca (Ian James Forsythe) at smtp
-: Date:    94/11/18 15:09
-
-
-: Can anyone tell me a good way of cleaning electroporation cuvettes for 
-: re-use?  Must get rid of all salt and also any residual DNA.
-:      
-: --
-: Ian J. Forsythe, Agriculture & Agrifood Canada
-: 6660 NW Marine Drive, Vancouver, BC V6T 1X2  Canada (Campus Zone 4) 
-: Telephone:  (604) 224-4355 local 229   Fax:  (604) 666-4994
-: e-mail:  ianjf at unixg.ubc.ca
-:      
-
-Just rinsing with water is insufficient provided you are using 
-electroporation to clone. Residual DNA will appear in subsequent 
-electroporations. Treat the cuvettes with 30% HNO3 for 1 hr after rinsing 
-with water. Then rinse with water again and fill with 95% EtOH. I just 
-let it dry down, but have also dried under vacuum after 24 hrs.
-
-
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-%                                                        #
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-%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#

A far less expensive and labor intensive method is to let them simply sit
under a germicidal UV source overnight after a thorough rinse. I have done
this for years and have never seen evidence of a residual DNA background.
Much kinder to the cuvettes, also.

Cuvette lifetimes are lengthened if you take pains not to scratch their
conductive inner surfaces. I generally fire polish the Pasteur pipette tip
before jamming it in. The incidence of arcing decreases considerably if
you follow this precaution.

Cheers,

Eric.



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