Antibody Production with synthetic Multiple Antigenic Peptides (MAPs)

Anne deVaux devauxae at ccmail.apldbio.com
Tue Nov 29 20:17:18 EST 1994


We are working on a project to raise antibodies to synthetic peptides using MAPs.  We have synthesized a 15 residue peptide on a 4-branch MAP construct.  This was used to immunize Balb/c mice and we obtained no antigenic response.  We think this may be due to a lack of a T-cell helper epitope.  Does anyone know of a universal T-cell epitope sequence compatible with Balb/c mice?  Or, have another explaination for why we obtained no response?

Anne deVaux & Jacqueline McCandless
devauxae at ccmail.apldbio.com



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