Nightmare of Baculovirus expression system
Jane E B Harrison
JEB.Harrison at auckland.ac.nz
Tue Nov 29 18:49:16 EST 1994
My Baculovirus Expression system is playing up and I can't get it to work again.
I thought it was the temperature at which the cells grew (one incubator
can be a bit variable), so I changed, and got some to grow our
recombinant protein. The next week I reapeat the same experiment usingthe
same batch of virus stock and the same conditions and NOTHING!
Any ideas/suggestions welcome.
I can't say what my protein is-sorry
Jane E B Harrison "Science is like being God-
Medical School only I have time and budget constraints"
University of Auckland
'All opinons are MINE and MINE alone'
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