regulation of tet promoter on pBR322???
patel_p at watson.bms.com
Wed Nov 30 09:51:00 EST 1994
For a specific use in a particular project I have constructed a home
made vector which is essentially like the pUC vectors except that I have
replaced the lac promoter with the tet promoter from pBR322.
The questions I have are:
1) Is the tet promoter of pBR regulated by a tet repressor??
2) If I "express" the tet repressor in the same cell will I be able to get
a "regulateble" induction of tet promoter with chloro-tetracycline
3) Does anybody know where I could get the tet repressor gene.
Any comments or suggestions will be appreciated.
Thanks in advance
e-mail: Patel_P at BMS.com
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