Pwo PCR: info wanted

PANIAGUA SOLIS JORGE FERNANDO jpaniagf at CONDOR.DGSCA.UNAM.MX
Wed Nov 30 18:23:13 EST 1994


Dear Fellows:
After succeding to amplify with Taq DNA Pol, I am trying to amplify a 1.3 
Kb band with Pwo DNA Pol, (Boehringer). I observed that, after ompimizing 
the Mg2SO4 concentration, instead of get the expected 1.3 Kb band, I got 
a 11-1.1 Kb band, and that the reaction is more temperature sensitive. Is 
thethere any one else that can share her/his experience with Pwo 
amplification?. Please reply to the e-mail, our internet connection is 
priprimitive and someone erased the bionet reading list. Thanks. 
jpaniagf at condor.dgsca.unam.mx



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