Methylation analysis/bisulphite sequencing

Jeffrey H. Boatright jboatri at eagle.cc.emory.edu
Wed Nov 30 16:54:51 EST 1994


We are trying to determine the methylation status of cytosine residues on
individual chromosomes by PCR amplifying genomic DNA that has been
bisulphite-treated, thus converting unmethylated cytosine residues into
uracil by deamination (Nuc. Acids Res. 22:695).

Our problem is that the bisulphite/hydroquinone treatment somehow causes
almost complete loss of starting DNA.  Is anyone attempting this and have
you met with success?

Thank you in advance for your advice/thoughts.

Jeff Boatright
Mach Bhati



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