Methylation analysis/bisulphite sequencing
Jeffrey H. Boatright
jboatri at eagle.cc.emory.edu
Wed Nov 30 16:54:51 EST 1994
We are trying to determine the methylation status of cytosine residues on
individual chromosomes by PCR amplifying genomic DNA that has been
bisulphite-treated, thus converting unmethylated cytosine residues into
uracil by deamination (Nuc. Acids Res. 22:695).
Our problem is that the bisulphite/hydroquinone treatment somehow causes
almost complete loss of starting DNA. Is anyone attempting this and have
you met with success?
Thank you in advance for your advice/thoughts.
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