cycle sequencing - dimensions
c2015 at rrzc5
Wed Nov 30 05:00:53 EST 1994
I'm planning to sequence a lot of PCR fragments for the project that I
just started. To simplify the work I want to avoid cloning.
So here some questions about cycle sequencing:
What are the first nucleotides that I'm able to read,
using the same primer (P33-labeled) that I used to amplify my fragment?
Using P33, how many bases am I able to read & is it possible to do a
long-run gel with that reaction?
Thanks for any help & it would be great if you could email me directly
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