cycle sequencing - dimensions

Birgit Aimer c2015 at rrzc5
Wed Nov 30 05:00:53 EST 1994


Hi,

I'm planning to sequence a lot of PCR fragments for the project that I
just started. To simplify the work I want to avoid cloning.
So here some questions about cycle sequencing:

What are the first nucleotides that I'm able to read,
  using the same primer (P33-labeled) that I used to amplify my fragment?

Using P33, how many bases am I able to read & is it possible to do a
  long-run gel with that reaction?

Thanks for any help & it would be great if you could email me directly

Mike




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