Optimum Mg concentration for Taq ligase & Taq polymerase

Yasuhiro NAITO ynaito at HAMA-MED.AC.JP
Wed Nov 30 12:31:00 EST 1994


  Hi Netters,

       I wonder why the optimum MgCl2 concentration for Taq ligase is
higher than that for Taq polymerase. 
       I think: These two enzymes work in cooperation. Thus they catalyze
in same environment. 

       Please tell me why.        
       Thanks a lot.

   ::;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;:;
   ::                                                        :;
   ::   Yasuhiro NAITO M.D.                                  :;
   ::                                                        :;
   ::   Department of Biochemistry, Hamamatsu Univ Sch Med   :;
   ::               3600 Handa-cho, Hamamatsu 431-31 Japan   :;
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   ::                  Internet: ynaito at hama-med.ac.jp       :;
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