Need help for emoving glycogen from DNA solution

Antonio Rodriguez-Ariza .bb1roara at lucano.uco.es
Wed Nov 30 07:44:56 EST 1994


I am having problems in the purification of total DNA from fish (Sparus
aurata, gilthead seabream) liver. Using two distinct protocols
(phenol/chloroform or benzylchloride extraction), I always obtain a DNA
solution but with a high amount of GLYCOGEN. How can I remove glycogen
from this DNA solution?
I would greatly appreciate receiving help.


Thanks



More information about the Methods mailing list